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+BE AWARE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and likewise a number of the amino teams within the spot areas do not form bonds with the amino acids. Make sure that all the purification buffers are at 4 °C and carry out the protein purification in the chilly room to keep away from protein denaturation. NOTICE: This avoids the coupling of amino acids within the further cycles with the membrane as a substitute of the growing peptide chain.
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